What would you do to a bacterial culture to get a 1/10 dilution plate?

What would you do to a bacterial culture to get a 1/10 dilution plate?

How would you inoculate a plate to get a 1:10 dilution? -Plate 0.1 ml of culture. –Mix 1 ml of a culture with 9 ml of water and plate 1 ml of the dilution. You just studied 8 terms!

Why is it necessary to perform a plate count in conjunction with the?

Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure? A plate count gives viable numbers while turbidimetry gives all particles, so living and dead cells.

How do you make a 10 by 6 dilution?

A 10-6 dilution can be achieved by making three 1:100 dilutions, or six 1:10 dilutions, or a combination of 100-fold and 10-fold dilutions. When following either of the above procedures, it must be remembered that in order to achieve a 10-6 dilution, 1.0 ml of sample from the last dilution must be plated.

When 1.0 ml of a sample is added to 9.0 ml of sterile media what is the dilution?

1. Add 1.0ml of the original broth culture to 9.0ml water (to obtain a 10-1dilution). The 10-1 dilution will have 600 cells/ml.

What would you do to a bacterial culture to get a 1/10 dilution plate 1/100 dilution quizlet?

How would you inoculate a plate to get a 1:10 dilution? AND a 1:100 dilution? Just plate 0.1ml for a 1:10. To do a 1:100 do a 1:10 dilution then plate 0.1 dilution then plate 0.1 ml

How do you dilute a bacterial culture?

A 10-6 dilution can be achieved by making three 1:100 dilutions, or six 1:10 dilutions, or a combination of 100-fold and 10-fold dilutions. When following either of the above procedures, it must be remembered that in order to achieve a 10-6 dilution, 1.0 ml of sample from the last dilution must be plated.

How would you prepare 10 ml of a 10 6 dilution of a bacterial culture?

A turbid broth tube contains millions of bacteria. If we transferred directly from that tube, we would get a plate that had a lawn of bacteria. This plate would be uncountable and we could not use it to estimate bacterial cell numbers. Therefore, we need to dilute our original sample before plating.

Why is it necessary to perform a plate count in conjunction with the spectrophotometric method?

Why is it necessary to perform a plate count in conjunction with the tubidimetry procedure? Because a plate count will be more accurate regarding the number of cells that are alive and growing.

What is the purpose of performing a standard plate count?

What is the purpose of the standard plate count? To estimate the bacterial population in a particular environment.

Why is it necessary to count only the plates with 25 to 250 colonies?

Ideally only plates with 25-250 colonies are used. Counts above 250 are considered Too Numerous To Count (TNTC) because it is impossible to tell whether colonies are separated. Plates with less than 25 colonies do not have a statistically significant number of colonies.

Why is the viable plate count technique considered?

A viable cell count allows one to identify the number of actively growing/dividing cells in a sample. The plate count method or spread plate relies on bacteria growing a colony on a nutrient medium. The colony becomes visible to the naked eye and the number of colonies on a plate can be counted.

How do you calculate a dilution ratio?

add the ratio numbers together. So for example: a dilution ratio of 4:1 would be 4+15 then I take the total ounces, which in this case is 32 and divide that by 5.How to calculate dilution ratios of 32 oz bottles?

  • 4:1 ratio in a 32oz bottle.
  • 4+1 5.
  • 32oz divided by 5 6.4oz.
  • Feb 19, 2020

    How do you do a 10 5 dilution?

    Answer: In this problem, the % solution is the number of grams solute in 100 ml solvent, so a 10% solution of NaCl is 10 grams NaCl in 100 ml water. But you need 500 ml, final volume, so 10 g x 5 50 g NaCl

    How do you do a simple dilution?

    For example, to make a 1:10 dilution of a 1M NaCl solution, you would mix one part of the 1M solution with nine parts of solvent (probably water), for a total of ten parts. Therefore, 1:10 dilution means 1 part + 9 parts of water (or other diluent).

    How do you calculate dilution in microbiology?

    To find a dilution of a single tube, use the formula: sample/(diluent + sample). The sample is the amount you are transferring into the tube, and the diluent is the liquid already in the tube. When you transfer 1 ml into 9 mls, the formula would be: 1/(1+9) 1/10. This could also be written as 1:10.

    How do you calculate serial dilution?

    In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. For example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, DFViVf 1mL10mL110 .

    What is the dilution method?

    The Dilution method is used to determine the minimal inhibitory concentration of an antimicrobial to inhibit or kill the bacteria/fungi and is the reference for antimicrobial susceptibility testing.

    How do you do a dilution in a lab?

    How much initial sample and diluent should you use? Answer: 1:5 dilution 1/5 dilution 1 part sample and 4 parts diluent in a total of 5 parts. If you need 10 ml, final volume, then you need 1/5 of 10 ml 2 ml sample. To bring this 2 ml sample up to a total volume of 10 ml, you must add 10 ml – 2 ml 8 ml diluent.

    What is the purpose of diluting a bacterial sample before plating it?

    How would you inoculate a plate to get a 1:10 dilution? -Plate 0.1 ml of culture. –Mix 1 ml of a culture with 9 ml of water and plate 1 ml of the dilution. You just studied 8 terms!

    Why do you Plate each dilution in triplicate?

    By diluting the sample, it is possible to plate different concentrations of the sample and obtain an incubate plate with an easily countable number of colonies and calculate the number of microbes present in the sample.

    How do you dilute a bacterial sample?

    Serial dilution is a process through which the concentration of an organism, bacteria in this example, is systematically reduced through successive resuspension in fixed volumes of liquid diluent. Usually the volume of the diluent is a multiple of 10 to facilitate logarithmic reduction of the sample organism.

    How do you dilute a culture?

    Cultural Dilution then can be defined as making predominating attitudes and behavior that characterize a group weaker or less concentrated.

    How is bacterial dilution calculated?

    How would you inoculate a plate to get a 1:10 dilution? -Plate 0.1 ml of culture. –Mix 1 ml of a culture with 9 ml of water and plate 1 ml of the dilution. You just studied 8 terms!

    When performing a standard plate count Why do we prefer counting colony forming units CFUs rather than individual bacterial cells?

    Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure? A plate count gives viable numbers while turbidimetry gives all particles, so living and dead cells.

    What is the advantage of standard plate count over an indirect method of counting bacteria?

    When performing a standard plate count, why are the counts reported as colony forming units (CFUs)? because one colony could grow form a group of cells.

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